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KMID : 1177720130070010023
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Journal of Alternatives to Animal Experiments
2013 Volume.7 No. 1 p.23 ~ p.27
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Anti-allergic effect of saccharides on hair dye precursor, p-toluenediamine sulfate, and its evaluation by in vitro alternative method based on ROS production in THP-1 cells
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Kim Ji-Hoon
Cho Sun-A
Choi Ji-Yei
Lee John-Hwan
Kim Jong-Hyub
Park Jae-Jung
Kim Sang-Hoon
Kim Wang-Gi
Shin Kye-Ho
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Abstract
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Allergic contact dermatitis (ACD) is undesired side effect caused by chemical allergens in the development of cosmetics, especially, hair coloring product. It has been reported that reactive oxygen species (ROS) produced by contact allergen plays a crucial role in dendritic cell maturation and contact hypersensitivity. In this study, we evaluated anti-allergic effect of various saccharides on hair dye precursor, p-toluenediamine sulfate (PTD) by use of ROS production in THP-1 cell. 80 μg/ml PTD showed significant increase in the level of ROS production in THP-1 cell. In addition, co-treatment with xylose or galactose showed dose-dependent ROS decrease at a range from 0.1 % to 1 %. Moreover, similar inhibition effect on PTD by xylose or galactose was also observed when the level of CD86 expression on THP-1 was evaluated. Base on these results, in vitro alternative method based on ROS production in THP-1 cells could be applied to screen the substances mitigating allergic contact dermatitis and xylose or galactose could be used in hair color product to reduce skin sensitization potential of aromatic amine hair dye precursors without any disturbing dyeing hair.
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KEYWORD
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Allergic contact dermatitis, Reactive oxygen species, Anti-allergic effect
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